Purification of CMP-N-acetylneuraminic acid synthetase from bovine anterior pituitary glands
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چکیده
منابع مشابه
Purification of pre-prolactin mRNA from bovine anterior pituitary glands.
Total cytoplasmic RNA, isolated from bovine anterior pituitary glands, directed the synthesis of primarily two proteins in a wheat germ cell-free system. Analysis of wheat germ cell-free products by sodium dodecyl sulfate-slab gel electrophoresis, direct immunoprecipitation with prolactin aatibody, and prolactin- and growth hormone-specific radioimmunoassay indicated that the two proteins were ...
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The gene encoding CIIP-A'-acet\ ' lneuraminic acid (CIIPNeuAc) s1'nthetase (EC 2.7.7.43) in Escherichia coli serot!'pe 07 Kl w'as isolated and overexpressed in E.coli W3ll0. \Iaximum expression of 8-1Ooh of the soluble E.coli protein rras achieved b1'placing the gene rr i th an engineered 5'-terminus and Shine-Dalgarno sequence into a pKK223 rector derivative behind the tac promoter. The overex...
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Adult male rats, under starving and normal conditions, were injected intravenously with N-acetyl[3H]mannosamine and after various time intervals the specific radioactivities of free N-acetylneuraminic acid (NeuAc) and CMP-N-acetylneuraminic acid were determined in the liver. The specific radioactivity of free NeuAc was high even within 20s after injection; the maximum was reached between 7 and ...
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The capsular polysaccharide is a critical virulence factor for group B streptococci associated with human infections, yet little is known about capsule biosynthesis. We detected CMP-Neu5Ac synthetase, the enzyme which activates N-acetylneuraminic acid (Neu5Ac, or sialic acid) for transfer to the nascent capsular polysaccharide, in multiple group B streptococcus serotypes, all of which elaborate...
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Fragile X mental retardation protein (FMRP), fragile X related 1 protein (FXR1P) and FXR2P are the members of the FMR protein family. These proteins contain two KH domains and a RGG box, which are characteristic of RNA binding proteins. The absence of FMRP, causes fragile X syndrome (FXS), the leading cause of hereditary mental retardation. FXR1P is expressed throughout the body and important f...
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ژورنال
عنوان ژورنال: Glycobiology
سال: 1999
ISSN: 1460-2423
DOI: 10.1093/glycob/9.5.481